Ac-IEPD-AFC

Product Name: Ac-IEPD-AFC

CAS No: 1135417-31-0

Purity: 95%

Molar Mass: 725.7

Chemical Formula: C32H38F3N5O11

Storage: Store at -20℃

Sequence: XEPD

Application: Ac-IEPD-AFC is a synthetic peptide substrate commonly used to study caspase-8 activity. This peptide sequence, acetyl-Ile-Glu-Pro-Asp, is linked to 7-amino-4-trifluoromethylcoumarin (AFC), a fluorescent reporter. Upon cleavage by caspase-8, AFC is released, emitting a fluorescent signal that can be quantitatively measured. This mechanism allows for precise monitoring of caspase-8 activity, which is crucial in apoptosis research. In pharmaceutical research, Ac-IEPD-AFC is utilized to screen potential inhibitors of caspase-8, aiding in the development of therapies for diseases involving apoptosis dysregulation, such as cancer and neurodegenerative disorders.

Reference: Briones, J. C., Espulgar, W. V., Koyama, S., Yoshikawa, H., Park, J., Naito, Y., … & Saito, M. (2020). A microfluidic platform for single cell fluorometric granzyme B profiling. Theranostics, 10(1), 123.